dnastar package Search Results


megalign 7 2 1  (DNASTAR)
99
DNASTAR megalign 7 2 1
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Megalign 7 2 1, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/MegAlign/pmc03973953-121-21-23
Average 99 stars, based on 1 article reviews
megalign 7 2 1 - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
lasergene genomic suite v 5 software package  (DNASTAR)
94
DNASTAR lasergene genomic suite v 5 software package
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Lasergene Genomic Suite V 5 Software Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/Lasergene+Genomics+Suite/pmc04959555-166-7-14
Average 94 stars, based on 1 article reviews
lasergene genomic suite v 5 software package - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
lasergene package  (DNASTAR)
99
DNASTAR lasergene package
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Lasergene Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/Lasergene/pm19913866-165-13-15
Average 99 stars, based on 1 article reviews
lasergene package - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
seqman  (DNASTAR)
97
DNASTAR seqman
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Seqman, supplied by DNASTAR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/SeqMan+Pro/10__1111_slash_j__1095___8339__2012__01326__x-103-5-6
Average 97 stars, based on 1 article reviews
seqman - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier
genequest program  (DNASTAR)
94
DNASTAR genequest program
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Genequest Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/GeneQuest/10__1074_slash_jbc__m010959200-146-10-14
Average 94 stars, based on 1 article reviews
genequest program - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
primerselect package  (DNASTAR)
96
DNASTAR primerselect package
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Primerselect Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/PrimerSelect/pm16466543-58-6-14
Average 96 stars, based on 1 article reviews
primerselect package - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
editseq  (DNASTAR)
96
DNASTAR editseq
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Editseq, supplied by DNASTAR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/EditSeq/10__1097_slash_qai__0b013e31819df3b3-50-30-31
Average 96 stars, based on 1 article reviews
editseq - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
dnastar software package  (DNASTAR)
97
DNASTAR dnastar software package
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Dnastar Software Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/SeqMan+NGen/pmc12777775-58-14-21
Average 97 stars, based on 1 article reviews
dnastar software package - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier
software package s seqbuilder module  (DNASTAR)
95
DNASTAR software package s seqbuilder module
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Software Package S Seqbuilder Module, supplied by DNASTAR, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/SeqBuilder+Pro/ppr0609355-29-2-1
Average 95 stars, based on 1 article reviews
software package s seqbuilder module - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
megalin program  (Lasergen Inc)
90
Lasergen Inc megalin program
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Megalin Program, supplied by Lasergen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/megalin+program/pmc04242230-97-20-23
Average 90 stars, based on 1 article reviews
megalin program - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
arraystar software package  (DNASTAR)
95
DNASTAR arraystar software package
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Arraystar Software Package, supplied by DNASTAR, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/ArrayStar/pm28759843-265-5-8
Average 95 stars, based on 1 article reviews
arraystar software package - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
protein weight matrix  (DNASTAR)
96
DNASTAR protein weight matrix
Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in <t>MegAlign</t> 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons
Protein Weight Matrix, supplied by DNASTAR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+package/MegAlign+Pro/pmc01557593-40-18-21
Average 96 stars, based on 1 article reviews
protein weight matrix - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in MegAlign 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons

Journal: Applied Microbiology and Biotechnology

Article Title: Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool

doi: 10.1007/s00253-013-5192-3

Figure Lengend Snippet: Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in MegAlign 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database. The phenogram was prepared using the MegAlign’s built-in phylogenetic tree function. The enzymes described in this study are indicated by a gray background (including the A. oryzae enzyme used for comparative purposes). The amino acid sequences with the following IDs were used: A. niger lacA ID XP_001389622, A. niger ID A2QL84, A. nidulans lacA ID XP_658360, A. nidulans lacB ID XP_658584, A. clavatus ID XP_001268843, A. fumigatus ID XP_752787, A. fumigatus ID XP_748360, A. oryzae ID XP_001727461, Penicilium sp. ID Q700S9, Trichoderma reesei CAD70669, Homo sapiens ID NP_000395, K. lactis XP_452194, K. lactis 3OB8_A, and Escherichia coli ID NP_414878. b SDS-PAGE (Tris–glycine; resolving gel: T12.5/C1, stacking gel: T5.7/C2.2) analysis of purified recombinant enzymes. Each galactosidase was obtained free from visible contaminations as judged by Coomassie Brilliant Blue staining. Purified recombinant proteins were incubated with (+) or without (−) PNGase F to verify whether they carry N -glycans. MW molecular weight in kilodaltons

Article Snippet: Fig. 1 Galactosidase phenogram and SDS-PAGE analysis of recombinant enzymes. a Protein alignment based on the ClustalW method was performed in MegAlign 7.2.1 (DNAstar Lasergene software package) using full-length protein sequences of galactosidases as obtained from the GenBank database.

Techniques: SDS Page, Recombinant, Software, Purification, Staining, Incubation, Molecular Weight